Filling gaps in the DNA barcode library - Aquatic insects (Ephemeroptera, Plecoptera, Trichoptera) from semi-mountainous and mountainous rivers of Ecoregion 6 (North Macedonia)
نویسندگان
چکیده
This research provides pivotal molecular genetic data on the community structure of aquatic insects from semi-mountainous and mountainous rivers 6 th Ecoregion that belongs to territory North Macedonia. The aim this is fill gaps for barcoding macroinvertebrates Balkan Peninsula check if existing barcode library could provide improved identifications specimens were not taxonomically determined lowest level possible. We analyzed 95 which total DNA was extracted COI region amplified sequenced. taxa selected 20 different localities western part species-level in order test efficiency methodology what missing library. From result one plate (95 specimens) we obtained: 16 samples without barodes, or failed 10 did have a match BOLD database. In remining 69 samples, three misidentified. barcoded species new fauna Macedonia, 11 are mayflies: Baetis melanonyx , Ecdyonurus vitoshensis E. macani ; stonefly Isoperla vjose caddisflies: Agapetus delicatulus Athripsodes bilineatus Glossosoma klotho Lepidostoma basale, Helicopsyche bacescui, Tinodes unicolor Odontocerum hellenicum . also four rarely found species: Zwicknia bifrons Drussus tenellus Hydropsyche botosaneanui bulbifera available as sp. SK2 (potential species). 83% barcoding, where some with low medium quality specimens, representatives genera Oxietyra Rhyacophila conclusion can confirm vouchers need be further identified by morphology added library, maybe we'll possibility describe well.
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ژورنال
عنوان ژورنال: ARPHA Conference Abstracts
سال: 2021
ISSN: ['2603-3925']
DOI: https://doi.org/10.3897/aca.4.e65053